Proline specific endopeptidase from Flavobacterium.
نویسندگان
چکیده
منابع مشابه
Proline-specific endopeptidase from Flavobacterium. Purification and properties.
Proline-specific endopeptidase (EC 3.4.21.26) was purified 1,400-fold in an overall yield of 10% from cell-free extracts of Flavobacterium meningosepticum by CMcellulose and hydroxyapatite column chromatographies and gel filtration on Sephadex G-150. The purified enzyme was apparently homogeneous as judged by both standard disc gel and sodium dodecyl sulfate (SDS)-gel electrophoresis. The enzy...
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Monospecific antiserum raised against rat brain proline endopeptidase is used to demonstrate the ubiquity of the enzyme and its unique role in the degradation of proline-containing peptides. All endoproteolytic activity directed toward proline residues in several rat tissues is shown to share one or more common antigenic determinants with rat brain proline endopeptidase. Similar activity from t...
متن کاملPatterns of extracellular proline-specific endopeptidases in Legionella and Flavobacterium spp. demonstrated by use of chromogenic peptides.
Some Legionella strains possess a strong extracellular proline-specific endopeptidase (PSE) activity. Using an enlarged selection of chromogenic peptides representing a variety of N-terminal amino-acids binding to a -prolyl-proline, paranitroanilide chain, PSE activity of Legionella and Flavobacterium strains was examined. Differences in PSE activity emphasized the importance of the chemical st...
متن کاملCharacterization of a prolyl endopeptidase from Flavobacterium meningosepticum. Complete sequence and localization of the active-site serine.
A prolyl endopeptidase was purified from Flavobacterium meningosepticum. It was digested with trypsin. Two oligonucleotides, based on tryptic peptide sequences and used in PCR experiments, amplified a 300-base pair (bp) fragment. A 2.4-kilobase EcoRI fragment that hybridized to the 300-bp probe was cloned in lambda ZAP and sequenced from both strands. It contains a reading frame of 2115 bp, enc...
متن کاملFlavobacterium hauense sp. nov., isolated from soil and emended descriptions of Flavobacterium subsaxonicum, Flavobacterium beibuense and Flavobacterium rivuli.
A strictly aerobic, Gram-staining-negative, rod-shaped, non-motile, yellow-pigmented bacterial strain, designated BX12(T), was isolated from soil collected from the peak area of Wudang Mountain in the city of Shiyan, Hubei province, China. Phylogenetic analysis of the 16S rRNA gene sequences showed that strain BX12(T) was most closely related to Flavobacterium subsaxonicum WB 4.1-42(T) (95.9% 1...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1978
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.42.2417